Oxygen evolution from hydrogen peroxide in photosystem II: flash-induced catalatic activity of water-oxidizing photosystem II membranes

Journal Article · Tue May 05 04:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00383a014· OSTI ID:5902543

Mano, J; Takahashi, M; Asada, K

Water-oxidizing photosystem (PS) II membranes treated with hydrogen peroxide in the dark showed backward transition of their S/sub 0/ state to the S/sub -1/ state, as deduced from the pattern of flash-dioxygen yields. In the presence of hydrogen peroxide, they evolved oxygen after the first flash, as reported for thylakoids. The authors used H/sub 2/ /sup 18/O/sub 2/ and a mass spectrometer that allowed monitoring of the oxygen isotopes in the medium to determine the flash yields. PS II membranes evolved /sup 18/O/sub 2/ and /sup 16/O/sub 2/ in H/sub 2/ /sup 18/O/sub 2/ below 1 mM, but little /sup 16/O/sub 2/ was evolved above 1 mM. No /sup 16/O/sup 18/O was evolved under any conditions. The oxygen yields from hydrogen peroxide at higher concentrations were more than 20-fold that for the PS II reaction center, and the decay of oxygen evolution after the flash was much slower than that from water. By shortening the dark interval between flashes, the evolution of /sup 18/O/sub 2/ was decreased and that of /sup 16/O/sub 2/ increased. Little /sup 18/O/sub 2/ was evolved under continuous light. The flash-induced evolution of dioxygen from hydrogen peroxide in PS II membranes is inferred from the assumption that the S/sub 0//S/sub 2/-state cycle has catalatic activity with a slow rate and that this activity is lost by the reduction of S/sub 0/ to the S/sub -1/ state, the spontaneous decay of S/sub 2/ to the S/sub 1/ state, and the advancement of S/sub 2/ to the S/sub 3/ state by light. Tris(hydroxymethyl)aminomethane (Tris) treatment of PS II membranes decreased the yield of dioxygen and extinguished the kinetic features that characterize the catalatic production of dioxygen from hydrogen peroxide.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 5902543
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:9; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
CATALYSIS
CHEMICAL REACTION YIELD
CHEMICAL REACTIONS
CHEMICAL STATE
ELEMENTS
EVEN-EVEN NUCLEI
FOOD
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INHIBITION
ISOTOPES
LIGHT NUCLEI
MASS SPECTROSCOPY
MEMBRANES
NONMETALS
NUCLEI
OXIDATION
OXYGEN
OXYGEN 16
OXYGEN 18
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
PEROXIDES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
SPECTROSCOPY
SPINACH
STABLE ISOTOPES
SYNTHESIS
VEGETABLES
YIELDS