Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Tyrosine phosphorylation of the insulin receptor is not required for receptor internalization: studies in 2,4-dinitrophenol-treated cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;

The relation between insulin-stimulated autophosphorylation of the insulin receptor and internalization of the receptor was studied in Fao rat hepatoma cells. Treatment of Fao cells with 2,4-dinitrophenol for 45 min depleted cellular ATP by 80% and equally inhibited insulin-stimulated receptor autophosphorylation, as determined by immunoprecipitation of surface-iodinated or (/sup 32/P)phosphate-labeled cells with anti-phosphotyrosine antibody. In contrast, internalization of the insulin receptor and internalization and degradation of /sup 125/I-labeled insulin by 2,4-dinitrophenol-treated cells were normal. These data show that autophosphorylation of the insulin receptor is not required for the receptor-mediated internalization of insulin in Fao cells and suggest that insulin receptor recycling is independent of autophosphorylation.

Research Organization:
Joslin Diabetes Center, Boston, MA (USA)
OSTI ID:
5900849
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 86:9; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of an endogenous substrate of the insulin receptor in cultured cells
Journal Article · Wed Jul 15 00:00:00 EDT 1987 · J. Biol. Chem.; (United States) · OSTI ID:6271746
Rapid internalization of the insulin receptor in rat hepatoma cells
Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6285671
Differences in the sites of phosphorylation of the insulin receptor in vivo and in vitro
Journal Article · Mon Aug 05 00:00:00 EDT 1985 · J. Biol. Chem.; (United States) · OSTI ID:6085870

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AROMATICS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL NEOPLASMS
GLANDS
HEPATOMAS
HORMONES
HYDROXY COMPOUNDS
INHIBITION
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
NEOPLASMS
NITRO COMPOUNDS
NITROPHENOL
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOLS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
RECEPTORS
SYNTHESIS
TRACER TECHNIQUES
TUMOR CELLS