Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides

Journal Article · Tue Jan 24 00:00:00 EST 1989 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00428a040· OSTI ID:5900582

Salvato, B; Giacometti, G M; Beltramini, M; Zilio, F; Giacometti, G; Magliozzo, R S; Peisach, J

The reaction of Octopus vulgaris hemocyanin with nitrite was studied under a variety of conditions in which the green half-met derivative is formed. Analytical evidence shows that the amount of chemically detectable nitrite in various samples of the derivative is not proportional to the cupric copper detected by EPR. The kinetics of oxidation of hemocyanin as a function of protein concentration and pH, in the presence of nitrite and ascorbate, is consistent with a scheme in which NO/sub 2/ is the reactive oxidant. We suggest that the green half-methemocyanin contains a metal center with one cuprous and one cupric copper without an exogenous nitrogen oxide ligand.

• Research Organization: Univ. of Padova (Italy)
• OSTI ID: 5900582
• Journal Information: Biochemistry; (United States), Vol. 28:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HEMOCYANIN
METABOLISM
NITRITES
BIOLOGICAL EFFECTS
NITROGEN OXIDES
BIOCHEMICAL REACTION KINETICS
ELECTRON SPIN RESONANCE
LIGANDS
MOLLUSCS
SPECTROPHOTOMETRY
ANIMALS
AQUATIC ORGANISMS
CHALCOGENIDES
INVERTEBRATES
KINETICS
MAGNETIC RESONANCE
METALLOPROTEINS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
REACTION KINETICS
RESONANCE
560300* - Chemicals Metabolism & Toxicology