Seed coat import and unloading in pisum. [Pisum sativum]

Conference · Sat Aug 01 04:00:00 EDT 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5900580

Grusak, M A; Minchin, P E.H.

Experiments were undertaken with empty, attached ovules of Pisum sativum to observe the effects of osmotic solution changes on seed coat import and unloading into the apoplast. Through the use of /sup 11/CO/sub 2/ pulse labelling along with collimated monitoring of plant sections, the authors were able to continuously and simultaneously measure total pod import, import into a single ovule, and washout from the ovule into a flow-through bathing solution. The authors results indicated that changes in bathing solution sucrose concentration had no immediate effect on tracer washout in Pisum, but did affect ovule import. Lowering the sucrose concentration decreased import and raising the concentration increased import. Furthermore, these import changes were only gradually reflected in the seed coat washout profile, suggesting a buffering capability of the non-phloem seed coat tissues. Additional results have also led them to propose that the terminal site of seed coat unloading in Pisum is the plasmalemma of an non-phloem seed coat cell type, that unloading from this site occurs via a passive membrane transport process, and that solutes move symplastically to this compartment from the phloem.

• Research Organization: DSIR, Lower Hutt, New Zealand
• OSTI ID: 5900580
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BUFFERS
CARBOHYDRATES
CARBON 11
CARBON ISOTOPES
DIFFUSION
DISACCHARIDES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEGUMINOSAE
LIGHT NUCLEI
MEMBRANE TRANSPORT
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OSMOSIS
PISUM
PLANTS
QUANTITY RATIO
RADIOISOTOPES
RHIZOBIUM
SACCHARIDES
SACCHAROSE
SEEDS
TRACER TECHNIQUES
TRANSLOCATION