Potentially lethal damage repair is due to the difference of DNA double-strand break repair under immediate and delayed plating conditions

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576979· OSTI ID:5899724

Frankenberg-Schwager, M; Frankenberg, D; Harbich, R

Cells plated immediately after irradiation on nutrient agar (immediate plating) exhibit a lower survival than cells which are kept under nongrowth conditions before plating (delayed plating). The difference between the survival curves obtained after immediate plating and delayed plating is considered to exhibit the cell's capacity to repair potentially lethal damage. In yeast evidence has been presented previously for the DNA double-strand break (DSB) as the molecular lesion involved in the repair of potentially lethal damage observed at the cellular level. Radiation-induced DSB are repaired in cells plated on nutrient agar, i.e., under growth conditions, as well as in cells kept under nongrowth conditions. In this paper DSB repair under growth and nongrowth conditions is studied with the help of the yeast mutant rad54-3 which is temperature conditional for DSB repair. It is shown that the extent of repair of potentially lethal damage can be varied by shifting the relative fractions of repair of DSB under growth conditions versus nongrowth conditions. Repair of DSB in cells plated on nutrient agar is promoted when glucose is substituted by Na-succinate as an energy source. As a result the immediate plating survival curve approaches the delayed plating survival curve, thus reducing the operationally defined repair of potentially lethal damage. We show that this reduced potentially lethal damage repair is caused, however, by a higher amount of DSB repair in cells immediately plated on succinate agar as compared to glucose agar.

• Research Organization: Gesellschaft fuer Strahlen-und Umweltforschung mbH, Frankfurt, Germany, F.R.
• OSTI ID: 5899724
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 111:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CELL DIVISION
COMPARATIVE EVALUATIONS
DNA
DNA REPAIR
FUNGI
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GLUCOSE
GROWTH
HEXOSES
IRRADIATION
LETHAL IRRADIATION
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIOINDUCTION
RECOVERY
REPAIR
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
SURVIVAL CURVES
YEASTS