Affinity of (/sup 14/C)nitrosopiperidine and metabolites for mouse epithelial tissues

Journal Article · Fri Dec 31 23:00:00 EST 1982 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(83)90249-1· OSTI ID:5899598

Marlowe, C; Waddell, W J

Male C57BL/6J mice were each administered iv 1.2 mg/kg (6.0 to 7.6 muCi) of (/sup 14/C)nitrosopiperidine ((/sup 14/C)NPIP) and frozen by immersion in dry ice/hexane at 0.1, 0.33, 1, 3, 9, and 24 hr after injection. The mice were processed for whole-body autoradiography without thawing or the use of any solvents; sagittal sections of the frozen mice were freeze-dried and placed on X-ray film to reveal areas of localization of radioactivity. The autoradiographs revealed intense localization of radioactivity at 6 min in the epithelium of the nose and bronchi, as well as in the liver, kidney, and salivary glands. There is virtually no affinity of (/sup 14/C)NPIP for melanin. Most of the same localizations persisted from 6 min through 24 hr. At 24 hr the most intense accumulation was in the epithelium of the bronchi, nose, salivary gland ducts, and esophagus as well as the liver and Harder's gland. The results are interpreted to suggest that at least one metabolite of NPIP which localizes in the sites where tumors occur may be similar to a metabolite of NNN. The distribution is consistent with metabolic conversion of (/sup 14/C)NPIP in liver and epithelium of nose and bronchi with subsequent localization of the metabolite in epithelium of esophagus and salivary gland ducts.

• Research Organization: Department of Pharmacology and Toxicology, School of Medicine, University of Louisville, Kentucky
• OSTI ID: 5899598
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 67:1; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BODY
BODY AREAS
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
DISTRIBUTION
EPITHELIUM
FACE
GLANDS
HEAD
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
MICE
NITROSO COMPOUNDS
NOSE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIPERIDINES
PYRIDINES
RESPIRATORY SYSTEM
RODENTS
SALIVARY GLANDS
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
VERTEBRATES