Increased survival of skin flaps by scavengers of superoxide radical

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5898446

Huang, L; Privalle, C T; Serafin, D; Klitzman, B

Elevation of rat abdominal skin flaps, followed by ligation and division of the left inferior neurovascular pedicle, resulted in only a 40% survival of the area normally perfused by the ligated artery and vein. Superoxide dismutase (SOD) (EC1.15.1.1) administered v (20,000 U/kg) 30 min before flap elevation increased survival to 52%. SOD derivatized with polyethylene gycol, which increases circulating half-life, was more effective, increasing survival to 80%. This protective effect resulted from the catalytic activity of the derivatized enzyme because inactivation by treatment with H/sub 2/O/sub 2/ eliminated its effect on skin flap survival. An equimolar mixture of Desferal and MnCl/sub 2/, which catalyzes the dismutation of O/sub 2//sup -/ in vitro, improved survival to 72%. Desferal-Fe/sup 3 +/, which lacks in vitro SOD activity, or Mn/sup 2 +/ alone did not affect the survival of skin flaps, but Desferal alone was nearly as effective as the Desferal-Mn/sup 2 +/ mixture. This effect of Desferal may result from acquisition of and subsequent removal of iron in vivo. These results support the view that the superoxide radical or a product derived from it plays a role in limiting the survival of island skin flaps.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 5898446
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 1:2; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BODY
CARDIOVASCULAR DISEASES
CHELATING AGENTS
DISEASES
ENZYMES
IRON COMPOUNDS
ISCHEMIA
MAMMALS
ORGANS
OXIDOREDUCTASES
PATHOGENESIS
PERFUSED TISSUES
RADICALS
RATS
RODENTS
SCAVENGING
SKIN
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VASCULAR DISEASES
VERTEBRATES