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Early stress proteins in soybean cells under anoxia. [Glycine max]

Conference · · Plant Physiol., Suppl.; (United States)
OSTI ID:5897704
This research examines the molecular effects of anaerobic stress on soybean cells. Soybean (Glycine max, var Keller) suspension cell cultures are incubated under controlled atmospheric flux having 21, 10, 2, and 0 kPa O/sub 2/ for 0 to 4 h and labelled with /sup 3/H-leucine for the last hour of treatment. Proteins are extracted and resolved by 2-D gels. Cell viability is determined by fluorescein diacetate stain. During the first hour of exposure to 10 kPa O/sub 2/, a pattern of three novel early stress proteins (ESP) having 28, 32 and 100 kD MW and 4.0-4.8 pI is detected by 2-D gels. As oxygen concentration decreases, two of these proteins cease to be expressed, while the third (32 kD) continues to be expressed for up to one hour under 0 kPa O/sub 2/. After two hours under anoxia, protein synthesis in soybean cell cultures essentially stops, while the cell viability still remains higher than 90%. Changes in the abundance of in vitro translation products of ESP-specific mRNAs during hypoxia will be reported.
Research Organization:
USDA-ARS, Columbus, OH
OSTI ID:
5897704
Report Number(s):
CONF-8707108-
Conference Information:
Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
Country of Publication:
United States
Language:
English