Emphysema model in rats treated intratracheally with elastase

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(87)80199-8· OSTI ID:5897511

Yokoyama, E; Nambu, Z; Uchiyama, I; Kyono, H

Pulmonary functions, morphology, and morphometry were examined in rats at 3, 7, and 10 weeks after a single intratracheal administration of 6.5 units of porcine pancreatic elastase in order to obtain a model of pulmonary emphysema which would be suitable for studying the responses of emphysematous lungs to atmospheric pollutants. Functional residual capacity and residual volume of the elastase-treated rats increased at all the times studied, but their total lung capacity increased only at 7 and 10 weeks compared with those of the saline-treated control rats. The increase in static lung compliance and the decrease in peak flow and maximum flow at 50% of total lung capacity during forced expiration were also observed in all except the 3-week elastase animals. The elastase-treated lungs showed morphological changes characteristic of emphysematous lesions. The increase in mean linear intercept length and the decrease in total alveolar surface area were demonstrated by these elastase-treated lungs. Based on these results, they conclude that an adequate and suitable model of pulmonary emphysemia could be obtained in rats 7-10 weeks after treatment with the present dose of elastase.

• Research Organization: National Institute of Industrial Health, Tokyo, Japan
• OSTI ID: 5897511
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 42:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BODY
DISEASES
EMPHYSEMA
FUNCTIONS
INTAKE
LUNGS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANS
POLLUTANTS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SINGLE INTAKE
TOXICITY
VERTEBRATES