Antigen-specific stimulation of amino acid transport in bovine lymphocytes

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041130219· OSTI ID:5896459

Tate, E H

Treatment of bovine lymphocytes isolated from animals which were either infected with Mycobacterium bovis or sensitized to a purified protein derivative (PPD-B) from this organism induced an increase in the transport of a-aminoisobutyric acid (AIB) and a-methylaminoisobutyric acid (MeAIB). PPD-B did not stimulate these transport activities in lymphocytes from nonsensitized animals. The transport stimulation was first measurable after about 7 hours of treatment, reached about a two-fold enhancement after 20 hours, and continued to increase to 30- to 40-fold after 6 days. The stimulation of AIB transport was inhibited by both ouabain and cycloheximide. Experiments to determine transport system specificities in nonstimulated lymphocytes showed that MeAIB transport was primarily by the Na/sup +/-dependent, A-system,and leucine transport was mostly by Na/sup +/-independent system(s). In contrast, AIB transport was about 25% by the A-system, 25% by at least one Na/sup +/-dependent, non-A-system, and 50% by one or more Na/sup +/-independent system(s). Analysis of the three components of AIB transport after treatment with PPD-B showed that: 1) transport by both the A-system and the Na/sup +/-independent system(s) was stimulated; 2) A-system transport was stimulated to a larger extent than Na/sup +/-independent transport; and 3) Na/sup +/-dependent, non-A-system transport was not stimulated significantly.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5896459
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 113; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATTLE
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DOMESTIC ANIMALS
DRUGS
FUNGICIDES
GLYCOSIDES
INHIBITION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
MICROORGANISMS
MITOGENS
MYCOBACTERIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
OUABAIN
PESTICIDES
PROTEINS
REAGENTS
RUMINANTS
SENSITIZERS
SOMATIC CELLS
STIMULATION
STROPHANTHINS
VERTEBRATES