Developmental regulation of human truncated nerve growth factor receptor

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Annals of Neurology; (USA)

DOI:https://doi.org/10.1002/ana.410290105· OSTI ID:5895488

DiStefano, P S; Clagett-Dame, M; Chelsea, D M; Loy, R ^[1]

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1. Abbott Laboratories, Abbott Park, IL (USA)

Monoclonal antibodies (designated XIF1 and IIIG5) recognizing distinct epitopes of the human truncated nerve growth factor receptor (NGF-Rt) were used in a two-site radiometric immunosorbent assay to monitor levels of NGF-Rt in human urine as a function of age. Urine samples were collected from 70 neurologically normal subjects ranging in age from 1 month to 68 years. By using this sensitive two-site radiometric immunosorbent assay, NGF-Rt levels were found to be highest in urine from 1-month old subjects. By 2.5 months, NGF-Rt values were half of those seen at 1 month and decreased more gradually between 0.5 and 15 years. Between 15 and 68 years, urine NGF-Rt levels were relatively constant at 5% of 1-month values. No evidence for diurnal variation of adult NGF-Rt was apparent. Pregnant women in their third trimester showed significantly elevated urine NGF-Rt values compared with age-matched normals. Affinity labeling of NGF-Rt with 125I-NGF followed by immunoprecipitation with ME20.4-IgG and gel autoradiography indicated that neonatal urine contained high amounts of truncated receptor (Mr = 50 kd); decreasingly lower amounts of NGF-Rt were observed on gel autoradiograms with development, indicating that the two-site radiometric immunosorbent assay correlated well with the affinity labeling technique for measuring NGF-Rt. NGF-Rt in urines from 1-month-old and 36-year-old subjects showed no differences in affinities for NGF or for the monoclonal antibody IIIG5. These data show that NGF-Rt is developmentally regulated in human urine, and are discussed in relation to the development and maturation of the peripheral nervous system.

• OSTI ID: 5895488
• Journal Information: Annals of Neurology; (USA), Journal Name: Annals of Neurology; (USA) Vol. 29:1; ISSN ANNED; ISSN 0364-5134
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CELL DIFFERENTIATION
DIAGNOSTIC TECHNIQUES
GROWTH FACTORS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
LABELLING
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MONOCLONAL ANTIBODIES
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES