The production of phosphatidylinositol trisphosphate is stimulated by thrombin in human platelets

Journal Article · Thu Jan 31 04:00:00 EST 1991 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(91)91448-L· OSTI ID:5895079

Nolan, R D; Lapetina, E G ^[1]

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1. Burroughs Wellcome Co., Research Triangle Park, NC (USA)

Untreated human platelets labeled to equilibrium with 32Pi contained undetectable levels of 3-phosphorylated phosphoinositides. Stimulation of platelets with thrombin for 5 min resulted in an enormous increase in the amount of phosphatidylinositol 3,4-bisphosphate. We now report that the levels of phosphatidylinositol 3,4,5-trisphosphate are greatly elevated within 90 s of treatment of platelets with thrombin. Phosphatidylinositol 3,4,5-phosphate might have an important role in platelet aggregation.

• OSTI ID: 5895079
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 174:2; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD PLATELETS
BODY FLUIDS
CHROMATOGRAPHY
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SERINE PROTEINASES
SYNTHESIS
THROMBIN
TRACER TECHNIQUES
VERTEBRATES