Protein phosphorylation: Localization in regenerating optic axons
- Cornell Univ. Medical College, New York, NY (USA)
A number of axonal proteins display changes in phosphorylation during goldfish optic nerve regeneration. (1) To determine whether the phosphorylation of these proteins was closely linked to their synthesis in the retinal ganglion cell body, cycloheximide was injected intraocularly into goldfish whose optic nerves had been regenerating for 3 weeks. Cycloheximide reduced the incorporation of (3H)proline and 32P orthophosphate into total nerve protein by 84% and 46%, respectively. Of the 20 individual proteins examined, 17 contained less than 15% of the (3H)proline label measured in corresponding controls, whereas 18 proteins contained 50% or more of the 32P label, suggesting that phosphorylation was largely independent of synthesis. (2) To determine whether the proteins were phosphorylated in the ganglion cell axons, axonal transport of proteins was blocked by intraocular injection of vincristine. Vincristine reduced (3H)proline labeling of total protein by 88% and 32P labeling by 49%. Among the individual proteins (3H)proline labeling was reduced by 90% or more in 18 cases but 32P labeling was reduced only by 50% or less. (3) When 32P was injected into the cranial cavity near the ends of the optic axons, all of the phosphoproteins were labeled more intensely in the optic tract than in the optic nerve. These results suggest that most of the major phosphoproteins that undergo changes in phosphorylation in the course of regeneration are phosphorylated in the optic axons.
- OSTI ID:
- 5894603
- Journal Information:
- Neurochemical Research; (USA), Vol. 15:9; ISSN 0364-3190
- Country of Publication:
- United States
- Language:
- English
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NERVE CELLS
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PHOSPHOPROTEINS
PHOSPHORYLATION
ANTINEOPLASTIC DRUGS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
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PHOSPHATES
PHOSPHORUS 32
PROLINE
RETINA
TRITIUM COMPOUNDS
TWO-DIMENSIONAL ELECTROPHORESIS
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AMINO ACIDS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EYES
FACE
HEAD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
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PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RECOVERY
SENSE ORGANS
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550501* - Metabolism- Tracer Techniques