Superiority of radiobinding assay over ELISA for detection of IAAs in newly diagnosed type I diabetic children

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Diabetes Care; (USA)

DOI:https://doi.org/10.2337/diacare.14.1.61· OSTI ID:5892467

Levy-Marchal, C; Bridel, M P; Sodoyez-Goffaux, F; Koch, M; Tichet, J; Czernichow, P; Sodoyez, J C ^[1]

---

1. Robert Debre Hospital, Paris (France)

Liquid- or solid-phase assays have been used for insulin autoantibody (IAA) determination, and the method of IAA measurement has not been standardized. IAAs were determined by radiobinding assay (RBA) and enzyme-linked immunosorbent assay (ELISA) in two large age-matched groups of nondiabetic and newly diagnosed insulin-dependent (type I) diabetic children. Positivity for IAA by RBA (greater than or equal to nondiabetic mean + 3SD) was 2 of 178 (1.1%) and 55 of 173 (32%) in nondiabetic and diabetic children, respectively. Prevalence of IAA by RBA was significantly higher in the youngest age-group (63% between 0-4 yr). Positivity for IAA by ELISA was 1 of 178 (0.6%) and 8 of 169 (4.7%) in nondiabetic and diabetic children, respectively. Concordance rates between both assays were 0 of 3 (0%) in control subjects and 5 of 58 (8.6%) in diabetic children. We conclude that RBA is more appropriate than ELISA for IAA detection at the onset of the disease. In addition, because available data suggest that IAAs detected by RBA only are high-affinity antibodies, it is tempting to speculate that IAAs reflect a mature immune reaction against endogenous insulin.

• OSTI ID: 5892467
• Journal Information: Diabetes Care; (USA), Vol. 14:1; ISSN 0149-5992
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
IMMUNOASSAY
DIABETES MELLITUS
PATHOGENESIS
AGE DEPENDENCE
CHILDREN
ENZYME IMMUNOASSAY
INSULIN
RADIOIMMUNOASSAY
AGE GROUPS
BIOASSAY
DIAGNOSTIC TECHNIQUES
DISEASES
ENDOCRINE DISEASES
HORMONES
IMMUNOLOGY
ISOTOPE APPLICATIONS
METABOLIC DISEASES
PEPTIDE HORMONES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
TRACER TECHNIQUES
550901* - Pathology- Tracer Techniques