Development and evaluation of the TD97 measles virus vaccine

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Journal of Medical Virology; (USA)

DOI:https://doi.org/10.1002/jmv.1890320311· OSTI ID:5892262

Suzuki, K; Morita, M; Katoh, M; Kidokoro, M; Saika, S; Yoshizawa, S; Hashizume, S; Horiuchi, K; Okabe, N; Shinozaki, T ^[1]

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1. Chiba Serum Institute (Japan)

The TD97 strain vaccine virus was prepared from the Tanabe strain measles virus by low-temperature passages in primary cell cultures and ultraviolet (UV) mutagenesis. The TD97 strain exhibited the following characteristics: highly temperature sensitive, neither multiplying nor forming any plaques at 40 degrees C in Vero cells; genetically stable, maintaining high temperature sensitivity after ten successive passages in CE cells at 30 degrees C or 35 degrees C; and M proteins of this virus about 1 KD slower in mobility in SDS-PAGE than that of the Tanabe strain. The TD97 strain was further confirmed to be attenuated by an inoculation test into primate brain. In field trials, 752 healthy children were inoculated with a live virus vaccine prepared with this strain, and the following results were obtained: the seroconversion rate was 97% (517/533), and the average HI antibody titer was 2(5.2). An antibody-increasing effect was also observed in children who were initially seropositive. In children who seroconverted, the rates of fever were 15.7% (55/351) for 37.5 degrees C or higher and 4.0% (14/351) for 39 degrees C or higher. The rash rate was 7.7% (27/351), and the incidence of local reaction was 5.4% (19/351). The TD97 strain is thus considered to be suitable in use for an attenuated measles vaccine.

• OSTI ID: 5892262
• Journal Information: Journal of Medical Virology; (USA), Journal Name: Journal of Medical Virology; (USA) Vol. 32:3; ISSN JMVID; ISSN 0146-6615
• Country of Publication: United States
• Language: English

Related Subjects

553003* -- Agriculture & Food Technology-- Pest & Disease Control-- (1987-)
60 APPLIED LIFE SCIENCES
AGE GROUPS
ANTIBODIES
CELL CULTURES
CHILDREN
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
INFECTIVITY
MICROORGANISMS
MUTAGENESIS
PARASITES
RADIATIONS
ULTRAVIOLET RADIATION
VACCINES
VIRAL DISEASES
VIRUSES