U1 small nuclear RNA genes are located on human chromosome 1 and are expressed in mouse-human hybrid cells

Journal Article · Thu Dec 01 04:00:00 EST 1983 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.3.12.2211· OSTI ID:5891922

Lund, E; Bostock, C; Robertson, M; Christie, S; Mitchen, J L; Dahlberg, J E

The majority, and perhaps all, of the genes for human U1 small nuclear RNA (U1 RNA) were shown to be located on the short arm of human chromosome 1. These genes were mapped by Southern blot analysis of DNA from rodent-human somatic cell hybrids, using the 5' region of a human U1 RNA gene as a human-specific probe. This probe hybridized to DNA fragments present only in digests of total human DNA or to the DNAs of cell lines which contained human chromosome 1. The major families of human U1 RNA genes were identified, but some human genes may have gone undetected. Also, the presence of a few U1 RNA genes on human chromosome 19 could not be ruled out. In spite of the lack of extensive 5'-flanking-region homology between the human and mouse U1 RNA genes, the genes of both species were efficiently transcribed in the hybrid cells, and the U1 RNAs of both species were incorporated into specific ribonucleoprotein particles.

• Research Organization: Dept. of Physiological Chemistry, Univ. of Wisconsin Medical School, Madison, WI 53706
• OSTI ID: 5891922
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 3:12; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CELL CONSTITUENTS
FUNCTIONS
GENETIC MAPPING
HYBRIDIZATION
MAMMALS
MAN
MAPPING
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANOIDS
PRIMATES
PROTEINS
RATS
RIBOSOMES
RNA
RNA PROCESSING
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES