Human DNA polymerase. alpha. : Predicted functional domains and relationships with viral DNA polymerases

Journal Article · Sat Dec 31 23:00:00 EST 1988 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5891082

Wang, T S.F.; Wong, S W; Korn, D ^[1]

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1. Stanford Univ., CA (United States)

The primary sequence of human DNA polymerase {alpha} deduced from the full-length cDNA contains regions of striking similarity to sequences in replicative DNA polymerases from Escherichia coli phages PRD1 and T4, Bacillus phage {phi}19, yeast DNA polymerase I, yeast linear plasmid pGKL1, maize S1 mitochondrial DNA, herpes family viruses, vaccinia virus, and adenovirus. The conservation of these homologous regions across this vast phylogenetic expanse indicates that these prokaryotic and eukaryotic DNA polymerases may all have evolved from a common primordial gene. Based on the sequence analysis and genetic results from yeast and herpes simplex virus studies, these consensus sequences are suggested to define potential sites that subserve essential roles in the DNA polymerase reaction. Two of these conserved regions appear to participate directly in the active site required for substrate deoxynucleotide interaction. One region toward the carboxyl-terminus has the potential to be the DNA interacting domain is predicted toward the amino-terminus. The provisional assignment of these domains can be used to identify unique or dissimilar features of functionally homologous catalytic sites in viral DBA polymerases of pathogenetic significance and thereby serve to guide more rational antiviral drug design.

• OSTI ID: 5891082
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 3:1; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EVOLUTION
CEREALS
COMPARATIVE EVALUATIONS
DNA POLYMERASES
DNA REPLICATION
DNA SEQUENCING
ENZYMES
ESCHERICHIA COLI
EUMYCOTA
EVALUATION
FUNGI
GENES
GRAMINEAE
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
MAMMALS
MAN
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
VIRUSES
YEASTS