Anaerobic degradation of soluble fractions of (/sup 14/C-lignin)lignocellulose
(/sup 14/C-lignin)lignocellulose was solubilized by alkaline heat treatment and separated into different molecular size fractions for use as the sole source of carbon in anaerobic enrichment cultures. This study is aimed at determining the fate of low-molecular-weight, polyaromatic lignin derivatives during anaerobic degradation. Gel permeation chromatography was used to preparatively separate the original /sup 14/C-lignin substrate into three component molecular size fractions, each of which was then fed to separate enrichment cultures. Biodegradability was assessed by monitoring total carbon dioxide and methane production, evolution of labeled gases, loss of /sup 14/C-activity from solution, and changes in gel permeation chromatographic elution patterns. Results indicated that the smaller the size of the molecular weight fraction, the more extensive the degradation to gaseous end products. In addition, up to 30% of the entire soluble lignin-derived carbon was anaerobically mineralized to carbon dioxide and methane.
- Research Organization:
- Stanford Univ., CA
- OSTI ID:
- 5889842
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 49:2; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
ALKANES
ANAEROBIC DIGESTION
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CARBON
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELLULOSE
CHALCOGENIDES
CHROMATOGRAPHY
DIGESTION
ELEMENTS
GEL PERMEATION CHROMATOGRAPHY
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
MANAGEMENT
METHANE
MOLECULAR WEIGHT
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROCESSING
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
WASTE MANAGEMENT
WASTE PROCESSING