Glutathione specifically labeled with isotopes

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(85)90464-6· OSTI ID:5889188

Murata, K; Abbott, W A; Bridges, R J; Meister, A

A procedure for synthesis of glutathione selectivity labeled with isotopes is described. A strain of Escherichia coli enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase by recombinant DNA techniques is immobilized in a carrageenan matrix and treated with toluene to render the cells more permeable to the substrates. The immobilized cell matrix is incubated with a mixture containing the appropriately labeled amino acid, the other amino acid constituents of glutathione, ATP, and acetylphosphate. The radiolabeled product is isolated by column chromatography.

• Research Organization: Cornell Univ. Medical College, New York, NY
• OSTI ID: 5889188
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Journal Issue: 1 Vol. 1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
BACTERIA
CHEMICAL PREPARATION
CHROMATOGRAPHY
DRUGS
ESCHERICHIA COLI
GLUTATHIONE
LABELLED COMPOUNDS
LABELLING
MICROORGANISMS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
SYNTHESIS