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Hydrolysis by commercial enzyme mixtures of AFEX-treated corn fiber and isolated xylans

Journal Article · · Applied Biochemistry and Biotechnology
DOI:https://doi.org/10.1007/BF02787986· OSTI ID:588703
; ;  [1];  [2]
  1. USDA Agricultural Research Service, Peoria, IL (United States)
  2. Texas A&M Univ., College Station, TX (United States)

Corn fiber is a coproduct produced during the corn wet-milling process and is similar to other high hemicellulose/cellulose-containing biomass such as grasses, straws, or bagasse, all of which represent potential fermentation feedstock for conversion into biofuels or other products. Corn fiber was subjected to ammonia-explosion (AFEX) treatment to increase degradability and then enzymatically digested with a combined mixture of commercial amylase, xylanase, and cellulose enzyme preparations. Whereas the starch and cellulose components were converted solely to glucose, oligosaccharides represented 30-40% of the xylan degradation products. This enzyme mixture also produced substantial oligosaccharides with xylans purified from corn fiber, corn germ, beech-wood, oatspelt, or wheat germ. Commercial xylan-degrading enzyme preparations containing xylanase, xylosidase, and arabinosidase activities were then used alone or in varying combinations to attempt to maximize degradation of these isolated xylans of differing chemical compositions. 25 refs., 5 tabs.

Sponsoring Organization:
USDOE
OSTI ID:
588703
Journal Information:
Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Journal Issue: 1 Vol. 62; ISSN 0273-2289; ISSN ABIBDL
Country of Publication:
United States
Language:
English