Peroxidase catalyzed polymerization of phenol

Journal Article · Mon Jul 01 00:00:00 EDT 1996 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02788061· OSTI ID:588697

Vasudevan, P T; Li, L O ^[1]

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1. Univ. of New Hampshire, Durham, NH (United States)

The effect of horseradish peroxidase (HRP) and H{sub 2}O{sub 2} concentrations on the removal efficiency of phenol, defined as the percentage of phenol removed from solution as a function of time, has been investigated. When phenol and H{sub 2}O{sub 2} react with an approximately one-to-one stoichiometry, the phenol is almost completely precipitated within 10 min. The reaction is inhibited at higher concentrations of H{sub 2}O{sub 2}. The removal efficiency increases with an increase in the concentration of HRP, but an increase in the time of treatment cannot be used to offset the reduction in removal efficiency at low concentrations of the enzyme, because of inactivation of the enzyme. One molecule of HRP is needed to remove approximately 1100 molecules of phenol when the reaction is conducted at pH 8.0 and at ambient temperature. 9 refs., 5 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 588697
• Journal Information: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Journal Issue: 1 Vol. 60; ISSN ABIBDL; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
54 ENVIRONMENTAL SCIENCES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOREACTORS
EFFICIENCY
ENZYME ACTIVITY
HYDROGEN PEROXIDE
PEROXIDASES
PH VALUE
PHENOL
POLYMERIZATION
PRECIPITATION
REDUCTION
REMOVAL
STOICHIOMETRY
TEMPERATURE DEPENDENCE
WASTE PROCESSING
WASTE WATER
WATER TREATMENT