Circular dichroism studies on single Chinese hamster cells

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00340a600· OSTI ID:5881844

Maestre, M F; Salzman, G C; Tobey, R A; Bustamante, C

The circular dichroism (CD) and circular intensity differential scattering (CIDS) contributions to the CD of single Chinese hamster (CHO) cells have been measured as a function of the position in the cell cycle. The data are analyzed in three main spectral regions: (1) the region above 290 nm (scattering region), (2) the 250-290-nm regions (nucleic acid absorption region), and (3) the region below 240 nm (protein absorption region). The results show that CD/CIDS microspectrophotometry is a good indicator of the cell cycle phase. The results are consistent with the view that chromatin is organized in chiral superstructures which differentially scatter circularly polarized light. These structures appear highly specific and repeatable as the cell passes through its cycle. 27 references, 5 figures.

• Research Organization: Lawrence Berkeley Lab., CA
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5881844
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 24:19; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL CYCLE
CHO CELLS
CHROMATIN
DATA
DICHROISM
EXPERIMENTAL DATA
INFORMATION
MAGNETIC CIRCULAR DICHROISM
MOLECULAR STRUCTURE
NUMERICAL DATA
SCATTERING
SPECTROPHOTOMETRY