Independence of buoyant cell density and growth rate in Escherichia coli

Journal Article · Sun Apr 01 04:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:5881842

Kubitchek, H E; Baldwin, W W; Schroeter, S J; Graetzer, R

The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth rate in any of three sets of experiments in which different gradient conditions were used. In addition, when cultures were allowed to enter the stationary phase of growth, mean cell volumes and buoyant densities usually remained unchanged for extended periods. These and earlier results support the existence of a highly regulated, discrete state of buoyant density during steady-state growth of E. coli and other cells that divide by equatorial fission. 11 references, 3 figures.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5881842
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 158:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELL DIVISION
CENTRIFUGATION
DATA
DENSITY
ESCHERICHIA COLI
EXPERIMENTAL DATA
GROWTH
INFORMATION
MICROORGANISMS
NUMERICAL DATA
PHYSICAL PROPERTIES
SEPARATION PROCESSES