Tetrahymena pyriformis cells are deficient in all mannose-P-dolichol-dependent mannosyltransferases but not in mannose-P-dolichol synthesis
Cells of the ciliated protozoan Tetrahymena pyriformis incubated with (/sup 14/C)glucose were found to synthesize Man-P-dolichol and Glc-P-dolichol, as well as Glc/sub 3/Man/sub 5/GlcNAc/sub 2/-P-P-dolichol, the latter being the main and largest lipid derivative formed. The missing mannose residues were those known to be transferred from Man-P-dolichol in other systems. Formation of Man-P-dolichol and of dolichol-P-P-oligosaccharides containing up to five mannose units was detected in cell-free assays containing protozoan membranes, rat liver dolichol-P, unlabeled Man/sub 4-9/GlcNAc/sub 2/-P-P-dolichol from pig liver, and GDP-(/sup 14/C)Man. Under exactly the same conditions but with UDP-(/sup 14/C)Glc instead of GDP-(/sup 14/C)Man, Glc-P-dolichol and dolichol-P-P-oligosaccharides containing five mannose and one to three glucose residues were formed in the absence of the pig liver compounds. In the presence of the latter, dolichol-P-P derivatives containing nine mannose and one to three glucose units were also synthesized. It is concluded that T. pyriformis cells are deficient in all Man-P-dolichol-dependent mannosyltransferases but not in Man-P-dolichol synthesis. The role of the latter compound in this microorganism is unknown.
- Research Organization:
- Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina
- OSTI ID:
- 5879371
- Journal Information:
- Biochemistry; (United States), Vol. 26:18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14 COMPOUNDS
BIOSYNTHESIS
HEXOSYL TRANSFERASES
BIOCHEMISTRY
OLIGOSACCHARIDES
CELL MEMBRANES
GLUCOSE
IN VITRO
LIVER
MANNOSE
RATS
SWINE
TETRAHYMENA
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CHEMISTRY
CILIATA
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENZYMES
GLANDS
GLYCOSYL TRANSFERASES
HEXOSES
INVERTEBRATES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PROTOZOA
RODENTS
SACCHARIDES
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques