Polyacrylamide gel miniaturization improves protein visualization and autoradiographic detection

Journal Article · Tue Feb 28 23:00:00 EST 1989 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(89)90053-5· OSTI ID:5879328

Mohamed, M A; Lerro, K A; Prestwich, G D

Polyacrylamide gels shrink to one-quarter of their original area when soaked in a 50% (w/v) solution of polyethylene glycol. Gel miniaturization improves the contrast of protein bands, with four valuable consequences. (i) A 5- to 10-fold increase in sensitivity for Coomassie blue is observed. (ii) Gels are more durable; i.e., they resist tearing when wet and they do not crack during drying under vacuum. (iii) Shrunken gels give sharper photographic images and provide better interlane protein band comparisons. (iv) Condensed protein bands lead to an increased sensitivity for detecting low-abundance, radioactively-labeled proteins by fluorography.

• Research Organization: State Univ. of New York, Stony Brook (USA)
• OSTI ID: 5879328
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 177:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALCOHOLS
ANIMALS
ARTHROPODS
AUTORADIOGRAPHY
ELECTROPHORESIS
GLYCOLS
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
ISOTOPES
LABELLED COMPOUNDS
LEPIDOPTERA
MOTHS
OPTIMIZATION
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYETHYLENE GLYCOLS
POLYMERS
PROTEINS
RADIOISOTOPES