Resistance of structure and antigenic determinations of native hexon of type 1 adenovirus to protease

Journal Article · Tue Feb 10 04:00:00 EST 1987 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5879218

Kiseleva, E K; Khil'ko, S N; Grigor'ev, V G; Dyachenko, N S; Vantsak, N P; Tikhonenko, T I

Native hexon capsomers (trimers) of human type 1 adenovirus (Ad hl) were labeled with /sup 125/I and subjected to cleavage by trypsin, chymotrypsin, and papain. The hydrolysis of the hexon of Ad 1 by these enzymes is limited and in each case a set of relatively large fragments, which are not cleaved during prolonged hydrolysis, is formed. The degree of hydrolysis of the Ad hl hexon increases in the order trypsin < chymotrypsin < papain, the molecular weight of the largest fragments constituting 80,000, 40,000, and 32,000, respectively. At a decreased temperature all the large fragments of the hydrolysates obtained are confined in aggregates (the cores of the hexon), similar in structure to the original hexon trimers, the papain core of the Ad hl hexon being the most stable during electrophoresis in polyacrylamide gel and the chymotrypsin core being the least. A radioimmunoprecipitation analysis showed that denatured fragments of the tryptic, chymotryptic, and papain hydrolysates do not possess antigenic activity.

• Research Organization: D.K. Zabolotnii Institute of Microbiology and Virology, Kiev, USSR
• OSTI ID: 5879218
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:8; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHYMOTRYPSIN
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
HALOGENATION
HYDROLASES
HYDROLYSIS
IMMUNE SERUMS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LYSIS
MAMMALS
MAN
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PAPAIN
PARASITES
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
PROTEOLYSIS
RABBITS
RADIOASSAY
RADIOENZYMATIC ASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SERINE PROTEINASES
SH-PROTEINASES
SOLVOLYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES
VIRUSES