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Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Oligosaccharide moieties of cell-surface glycoconjugates are thought to be involved in recognition events associated with tumor metastasis and invasion. Using swainsonine (SW), an inhibitor of Golgi ..cap alpha..-mannosidase II that results in the formation of hybrid-type oligosaccharides on N-linked glycoproteins, the authors have tested the hypothesis that specific glycan structures are required for pulmonary colonization by tumor cells. B16-F10 murine melanoma cells were treated with SW in growth medium and then injected intravenously into syngeneic C57BL/6 mice. This treatment resulted in dramatic inhibition of colonization, but it had no effect on B16-F10 viability or on cellular tumorigenicity after subcutaneous implantation. SW-treated radiolabeled B16-F10 cells were cleared from the lungs at a great rate than control cells, suggesting that one effect of treatment is to alter tumor cell retention in the target organ. The results implicate specific glycan structures in pulmonary colonization and offer a potential approach for identification of specific macromolecules involved in tumor cell-organ recognition during metastasis.
Research Organization:
Howard Univ. Cancer Center, Washington, DC
OSTI ID:
5878767
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
COLONY FORMATION
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
LUNGS
MAMMALS
MELANOMAS
MEMBRANES
METASTASES
MICE
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
URACILS
VERTEBRATES