Mutants of PC12 cells with altered cyclic AMP responses

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.10.2091· OSTI ID:5877922

Block, T; Kon, C; Breckenridge, B M

PCl2 cells, derived from a rat pheochromocytoma, were mutagenized and selected in media containing agents known to elevate intracellular concentrations of cyclic AMP (cAMP). More than 40 clones were isolated by selection with cholera toxin or 2-chloroadenosine or both. The variants that were deficient in accumulating cAMP were obtained by using a protocol in which 1 ..mu..m 8-bromo-cAMP was included in addition to the agonist. Certain of these variants were partially characterized with respect to the site of altered cAMP metabolism. The profiles of adenylate cyclase activity responsiveness of certain variants to guanosine-5'-(BETA,..gamma..-imido) triphosphate and to forskolin resembled those of UNC and cyc phenotypes of S49 lymphoma cells, which are functionally deficient in the GTP-sensitive coupling protein, N/sub s/. Other variants were characterized by increased cyclic nucleotide phosphodiesterase activity at low substrate concentration. Diverse morphological traits were observed among the variants, but it was not possible to assign them to a particular cAMP phenotype. Two revertants of a PCl2 mutant were isolated and observed to have regained a cellular cAMP response to 2-chloroadenosine and to forskolin. It is hoped that these PCl2 mutants will have utility for defining cAMP-mediated functions, including any links to the action of nerve growth factor, in cells derived from the neural crest.

• Research Organization: Dept. of Pharmacology, Univ. of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway, NJ 08854
• OSTI ID: 5877922
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:10; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ADENYLIC ACID
ADRENAL GLANDS
AMP
ANIMAL CELLS
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
BACTERIAL DISEASES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CELL CULTURES
CHLORINE COMPOUNDS
CHOLERA
CULTURE MEDIA
CYCLASES
DEFECTS
DISEASES
ENDOCRINE GLANDS
ENZYME INDUCTION
ENZYMES
ESTERASES
GENE REGULATION
GLANDS
GUANOSINE
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INFECTIOUS DISEASES
LYASES
LYMPHOMAS
MAMMALS
MATERIALS
MUTANTS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHENOTYPE
PHOSPHATES
PHOSPHODIESTERASES
PHOSPHORUS COMPOUNDS
PHYSIOLOGY
PURINES
RATS
RIBOSIDES
RODENTS
SYNTHESIS
TOXIC MATERIALS
TOXINS
TUMOR CELLS
VERTEBRATES