In vitro technique to study elution of benzo(a)pyrene from particulates into biomembranes with application to woodstove particulates

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(85)90446-4· OSTI ID:5877594

Bevan, D R; Yonda, N T

A method to study elution of benzo(a)pyrene (B(a)P) from carbonaceous particulates into phospholipid vesicles composed of dimyristoyl phosphatidylcholine (DMPC) was developed. Recoveries of B(a)P at each step of the procedure were quantified, with overall recoveries being 81%. Application of the technique to elution of B(a)P from woodstove particulate revealed that 25% of B(a)P associated with the particulate was eluted into DMPC vesicles in 18 h. From data obtained, it also was possible to evaluate elution of another polycyclic aromatic hydrocarbon (PAH), benzo(k)fluoranthene (B(k)F). Of the total B(k)F associated with woodstove particulate, 68% was eluted. This technique provides a convenient method for evaluating the potential bioavailability of PAH associated with other carbonaceous particulates which occur in the environment.

• Research Organization: Virginia Polytechnic Institute and State Univ., Blacksburg
• OSTI ID: 5877594
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZOPYRENE
BIOLOGICAL AVAILABILITY
CHROMATOGRAPHY
COMBUSTION PRODUCTS
CONDENSED AROMATICS
ESTERS
HEATING
HYDROCARBONS
IN VITRO
LIPIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARTICLES
PARTICULATES
PHOSPHOLIPIDS
SEPARATION PROCESSES
SOLUBILITY
WOOD