Nuclear Overhauser effect studies on the conformation of magnesium adenosine 5'-triphosphate bound to rabbit muscle creatine kinase
Nuclear Overhauser effects were used to determine interproton distances on MgATP bound to rabbit muscle creatine kinase. The internuclear distances were used in a distance geometry program that objectively determines both the conformation of the bound MgATP and its uniqueness. Two classes of structures were found that satisfied the measured interproton distances. Both classes had the same anti glycosidic torsional angle (X = 78 +/- 10/sup 0/) but differed in their ribose ring puckers (O1'-endo or C4'-exo). The uniqueness of the glycosidic torsional angle is consistent with the preference of creatine kinase for adenine nucleotides. One of these conformations of MgATP bound to creatine kinase is indistinguishable from the conformation found for Co(NH/sub 3/)/sub 4/ ATP bound to the catalytic subunit of protein kinase, which also has a high specificity for adenine nucleotides. Distance geometry calculations also suggest that upper limit distances, when low enough (less than or equal to 3.4 A), can be used instead of measured distances to define, within experimental error, the glycosidic torsional angle of bound nucleotides. However, this approach does not permit an evaluation of the ribose ring pucker.
- Research Organization:
- Univ. of Texas Medical School, Houston
- OSTI ID:
- 5876797
- Journal Information:
- Biochemistry; (United States), Vol. 26:17
- Country of Publication:
- United States
- Language:
- English
Similar Records
NMr studies of the AMP binding site and mechanism of adenylate kinase
Quantification of DNA structure from NMR data: Conformation of d-ACATCGATGT
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PHOSPHOTRANSFERASES
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
ATP
CATIONS
CHEMICAL SHIFT
COMPLEXES
CREATINE
GEOMETRY
MAGNESIUM COMPOUNDS
MUSCLES
OVERHAUSER EFFECT
PROTONS
RABBITS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BARYONS
CARBOXYLIC ACIDS
CHARGED PARTICLES
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
IONS
MAGNETIC RESONANCE
MAMMALS
MATHEMATICS
NUCLEONS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RESONANCE
TRANSFERASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques