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Nuclear Overhauser effect studies on the conformation of magnesium adenosine 5'-triphosphate bound to rabbit muscle creatine kinase

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00391a018· OSTI ID:5876797
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Nuclear Overhauser effects were used to determine interproton distances on MgATP bound to rabbit muscle creatine kinase. The internuclear distances were used in a distance geometry program that objectively determines both the conformation of the bound MgATP and its uniqueness. Two classes of structures were found that satisfied the measured interproton distances. Both classes had the same anti glycosidic torsional angle (X = 78 +/- 10/sup 0/) but differed in their ribose ring puckers (O1'-endo or C4'-exo). The uniqueness of the glycosidic torsional angle is consistent with the preference of creatine kinase for adenine nucleotides. One of these conformations of MgATP bound to creatine kinase is indistinguishable from the conformation found for Co(NH/sub 3/)/sub 4/ ATP bound to the catalytic subunit of protein kinase, which also has a high specificity for adenine nucleotides. Distance geometry calculations also suggest that upper limit distances, when low enough (less than or equal to 3.4 A), can be used instead of measured distances to define, within experimental error, the glycosidic torsional angle of bound nucleotides. However, this approach does not permit an evaluation of the ribose ring pucker.

Research Organization:
Univ. of Texas Medical School, Houston
OSTI ID:
5876797
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:17; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
ATP
BARYONS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL SHIFT
COMPLEXES
CREATINE
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
GEOMETRY
HADRONS
IONS
MAGNESIUM COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
MATHEMATICS
MOLECULAR STRUCTURE
MUSCLES
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTONS
RABBITS
RESONANCE
TRANSFERASES
VERTEBRATES