2',3'-dideoxythymidine 5'-triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis in human cells: evidence for involvement of DNA polymerase delta
It is well established that DNA replication and ultraviolet-induced DNA repair synthesis in mammalian cells are aphidicolin-sensitive and thus are mediated by one or both of the aphidicolin-sensitive DNA polymerases, ..cap alpha.. and/or delta. Recently, it has been shown that DNA polymerase delta is much more sensitive to inhibition by the nucleotide analogue 2',3'-dideoxythymidine 5'-triphosphate (ddTTP) than DNA polymerase ..cap alpha.. but is less sensitive than DNA polymerase BETA. The authors find that DNA replication and ultraviolet-induced DNA repair synthesis in permeable human fibroblasts are also more sensitive to inhibition by ddTTP than polymerase ..cap alpha.. and less sensitive than polymerase ..beta... The K/sub i/ for ddTTP of replication is about 40 ..mu..M and that of repair synthesis is about 25 ..mu..M. These are both much less than the K/sub i/ of polymerase ..cap alpha.. (which is greater than 200 ..mu..M but greater than the K/sub i/ of polymerase ..beta.. (which is less than 2 ..mu..M). These data suggest that DNA polymerase delta participates in DNA replication and ultraviolet-induced DNA repair synthesis in human cells.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO
- OSTI ID:
- 5874558
- Journal Information:
- Biochemistry; (United States), Vol. 26:10
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of DNA polymerase inhibitors on DNA repair in intact and permeable human fibroblasts: Evidence that DNA polymerases. delta. and. beta. are involved in DNA repair synthesis induced by N-methyl-N prime -nitro-N-nitrosoguanidine
In situ enzymology of DNA replication and ultraviolet-induced DNA repair synthesis in permeable human cells
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
BIOCHEMICAL REACTION KINETICS
DNA REPAIR
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
DNA REPLICATION
HELA CELLS
NUCLEOTIDES
PHOSPHORUS 32
ULTRAVIOLET RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RECOVERY
REPAIR
TRANSFERASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques