Preliminary studies of radiolabeled monoclonal antibody lymphoscintigraphy in malignant melanoma

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:5873983

Eary, R F; Jones, R; Kishore, R; Krohn, K A; Beaumier, P; Hellstrom, I; Hellstrom, K E

Studies have been initiated to examine the ability of I-131 anti-melanoma antibody (Fab) to delineate metastases in regional lymp nodes. 500 ..mu..gm of I-131 Fab (48.7) directed against HMW antigen of melanoma and I-125 non-specific Fab were injected subcutaneously distal to sites of node resection in 5 patients 20 hours prior to surgery. 3 additional injections were made distal to non-diseased inguinal nodes. 3 pts had no palpable nodes (class 1) and 2 did (class II). Gamma imaging of nodes at 3 and 18 hrs was negative in non-diseased areas and was positive in Pts 1-3; Pt 1 had only micrometastases occupying less than 1% of node volume. In-vitro counting of nodes showed concentrations of I-131 of % .0015 dose/gm of whole node and presumably much higher on tumor cells per se. Autoradiography of I-131 specific Fab in tumored nodes showed high discrete concentration on tumor compared to normal areas. Autoradiography of non-specific I-125 is currently underrway. The authors conclude these preliminary data show considerable promise for pre-surgical delineation of metastatic disease by radiommuno- lymphoscintigraphy.

• Research Organization: Oncogen and Univ. of Washington, Seattle, WA
• OSTI ID: 5873983
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DISTRIBUTION
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPES
LABELLING
LYMPH NODES
LYMPHATIC SYSTEM
MELANOMAS
METASTASES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
TISSUE DISTRIBUTION
TUMOR CELLS
UPTAKE
USES