Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Stimulation of ribosomal protein S6 kinase activity by pp60/sup v-src/ or by serum: dissociation from phorbol ester-stimulated activity

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;
Ribosomal protein S6 kinase activity was measured in lysates prepared from serum-deprived chicken embryo fibroblasts (CEF) treated for various times with phorbol 12-myristate 13-acetate (PMA). Maximal activity was observed within 15 min, and it declined to the initial level by 4 hr. Incubation of these cells with PMA 4-60 hr after the initial treatment did not result in an additional increase in S6 protein kinase activity. These results are consistent with down-regulation of the PMA receptor, protein kinase C, and the dependence of PMA-stimulated S6 kinase activity on this enzyme. Long-term pretreatment of CEF with PMA only partially attenuated the stimulation of the S6 protein kinase activity by serum or by expression of the Rous sarcoma virus transforming gene product, pp60/sup v-src/. A similar protein kinase activity also was stimulated in cells treated with cycloheximide or sodium vanadate. Pretreatment with PMA had little effect on this response. These data indicate that it is likely that there are at least two mechanisms through which S6 kinase activity can be regulated, one of which apparently utilizes protein kinase C whereas the other(s) does not. Additional experiments show PMA-stimulated glucose transport was not attenuated by long-term incubation with phorbol ester, suggesting that another mechanism, which is not dependent on the presence of protein kinase C, maintains this response after the proposed down-regulation of the PMA receptor.
Research Organization:
Harvard Univ., Cambridge, MA
OSTI ID:
5873610
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:6; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of a phorbol ester-stimulated S6 kinase from MDCK renal epithelial cells
Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6015402
Differential role of protein kinase C in desensitization of muscarinic receptor induced by phorbol esters and receptor agonists
Thesis/Dissertation · Sat Dec 31 23:00:00 EST 1988 · OSTI ID:6612860
Stimulation of dopamine synthesis and activation of tyrosine hydroxylase by phorbol diesters in rat striatum
Journal Article · Sun Mar 22 23:00:00 EST 1987 · Life Sci.; (United States) · OSTI ID:6934983

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIRDS
BLOOD SERUM
CARCINOGENS
CELL CONSTITUENTS
CHICKENS
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
CYTOLOGICAL TECHNIQUES
DRUGS
EMBRYONIC CELLS
ENZYME ACTIVITY
ENZYMES
ESTERS
FIBROBLASTS
FOWL
FUNGICIDES
KINETICS
MEMBRANE PROTEINS
MICROORGANISMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PARASITES
PESTICIDES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROMOTERS
PROTEINS
REACTION KINETICS
RECEPTORS
RIBOSOMES
SODIUM COMPOUNDS
SOMATIC CELLS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
TUMOR PROMOTERS
VANADATES
VANADIUM COMPOUNDS
VERTEBRATES
VIRUSES