Recent progress in cell-free solubilization of coal
Low rank coal has been solubilized using cell-free filtrates separated from cultures of Polyporus versicolor. Solubilization has been obtained with neat filtrates and with fractions collected from the neat filtrates after gel permeation chromatography. The coal solubilizing enzymes have been collected in enriched fractions with gpc. This increased relative purity has allowed the determination of the average molecular weight of this enzyme by gel permeation chromatography and by polyacrylamide gel electrophoresis. Rates of coal solubilization are dependent on the size of coal particles, mass of coal, temperature, pH, concentration of the cell-free filtrate, and the concentration of several inorganic ions.
- Research Organization:
- Univ. of Hartford, CT (USA)
- OSTI ID:
- 5871445
- Report Number(s):
- CONF-8809269-
- Journal Information:
- Prepr. Pap., Am. Chem. Soc., Div. Fuel Chem.; (United States), Vol. 33:4; Conference: Bioprocessing of coal, Los Angeles, CA, USA, 25-30 Sep 1988
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
COAL
DISSOLUTION
POLYPORUS VERSICOLOR
ENZYME ACTIVITY
AMMONIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
COAL EXTRACTS
ENZYMES
FRACTIONATION
GEL PERMEATION CHROMATOGRAPHY
IRON
MOLECULAR WEIGHT
PARTICLE SIZE
PH VALUE
PHOSPHATES
QUANTITY RATIO
SEPARATION PROCESSES
TEMPERATURE DEPENDENCE
CARBONACEOUS MATERIALS
CHROMATOGRAPHY
ELEMENTS
ENERGY SOURCES
FOSSIL FUELS
FUELS
FUNGI
KINETICS
MATERIALS
METALS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
REACTION KINETICS
SIZE
TRANSITION ELEMENTS
010402* - Coal
Lignite
& Peat- Purification & Upgrading
550700 - Microbiology