Vacuolar accumulation of heavy metals in Datura cultured cells is metal concentration dependent

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5870681

Krotz, R M; Wagner, G J

Vacuolar-extravacuolar compartmentation studies were performed to determine if the vacuole serves as an accumulation site for Cd, Zn, and Ni, after growth of Datura cultured cells in trace and high levels of these metals. After 3 to 4 days growth with 0.12 ..mu..M Cd or 0.02 ..mu..M Ni (radiolabeled) no evidence was obtained for vacuolar accumulation of these metals. In contrast, growth with 30 or 45 ..mu..M Cd, 11 ..mu..M Ni (with or without trace radiolabel), or 300 and 500 ..mu..M Zn resulted in isolated vacuoles which were enriched in metal. Compartmentation after exposure to low levels of Zn and also Cu is being investigated as is the subcellular site(s) of Cd-binding peptide formed during growth in high Cd. The hypothesis that Zn is accumulated as vacuolar organic acid salts is being tested directly because no evidence was found for formation of substantial ligand of Cd-peptide in response to Zn exposure.

• Research Organization: Univ. of Kentucky, Lexington
• OSTI ID: 5870681
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL LOCALIZATION
CADMIUM
ELEMENTS
GROWTH
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
METALS
NICKEL
ORGANIC COMPOUNDS
PEPTIDES
PLANT CELLS
PLANT GROWTH
PROTEINS
REACTION KINETICS
TRACE AMOUNTS
TRACER TECHNIQUES
TRANSITION ELEMENTS
ZINC