Correlation between changes in light energy distribution and changes in thylakoid membrane polypeptide phosphorylation in Chlamydomonas reinhardtii
We have used a new method to extensively modify the redox state of the plastoquinone pool in Chlamydomonas reinhardtii intact cells. This was achieved by an anaerobic treatment that inhibits the chlororespiratory pathway recently described by P. Bennoun. A state I (plus 3,4-dichlorophenyl-1,1-dimethylurea) ..-->.. anaerobic state transition induced a decrease in the maximal fluorescence yield at room temperature and in the F/sub PSII//F/sub PSI/ ratio at 77/sup 0/K, which was three times larger than in a classical state I ..-->.. state II transition. The fluorescence changes observed in vivo were similar in amplitude to those observed in vitro upon transfer to the light of dark-adapted, broken chloroplasts incubated in the presence of ATP. We then compared the phosphorylation pattern of thylakoid polypeptides in C. reinhardtii in vitro and in vivo using ..gamma..-(/sup 32/P)ATP and (/sup 32/P)orthophosphate labeling, respectively. The same set of polypeptides, mainly light-harvesting complex polypeptides, was phosphorylated in both cases. We observed that this phosphorylation process is reversible and is mediated by the redox state of the plastoquinone pool in vivo as well as in vitro. Similar changes of even larger amplitude were observed with the F34 mutant intact cells lacking in photosystem II centers. The presence of the photosystem II centers is then not required for the occurrence of the plastoquinone-mediated phosphorylation of light-harvesting complex polypeptides.
- Research Organization:
- Institut de Biologie Physico-Chimique, Paris, France
- OSTI ID:
- 5863013
- Journal Information:
- J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 98:1; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
AMIDES
ANAEROBIC CONDITIONS
AROMATICS
ATP
BENZOQUINONES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBONIC ACID DERIVATIVES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLAMYDOMONAS
CHLORINATED AROMATIC HYDROCARBONS
CHLOROPLASTS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
FLUORESCENCE
HALOGENATED AROMATIC HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LUMINESCENCE
MEMBRANES
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
PLASTOQUINONE
POLYPEPTIDES
PROTEINS
QUINONES
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
UNICELLULAR ALGAE
UREA
VISIBLE RADIATION