Comparison of Limulus assay, standard plate count, and total coliform count for microbiological assessment of renovated wastewater

Journal Article · Tue May 01 00:00:00 EDT 1979 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5859244

Jorgensen, J H; Lee, J C; Alexander, G A; Wolf, H W

The Limulus endotoxin assay was compared to the standard plate count and total coliform count for assessment of the bacteriological quality of reclaimed wastewater. A total of 48 water samples from an advanced waste treatment plant in Dallas, Texas, were examined by the three techniques. Limulus assays were technically simpler to perform and provided results much sooner than conventional culture methods. However, the endotoxin values did not correlate extremely well with determinations of viable bacterial numbers. This lack of correlation may have been due to alterations in the normal ratio of viable gram-negative cells to endotoxin caused by water reclamation procedures.

• Research Organization: Univ. of Texas, San Antonio
• OSTI ID: 5859244
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 37:5
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
COLIFORMS
BIOASSAY
PURIFICATION
RECYCLING
HEALTH HAZARDS
WASTE WATER
HAZARDS
HYDROGEN COMPOUNDS
LIQUID WASTES
OXYGEN COMPOUNDS
WASTES
WATER
520500* - Environment
Aquatic- Site Resource & Use Studies- (-1989)
320305 - Energy Conservation
Consumption
& Utilization- Industrial & Agricultural Processes- Industrial Waste Management
570000 - Health & Safety