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Title: Diverse effects of three furocoumarins on human lymphocyte proliferation

Abstract

The biological effects of three furocoumarins on the proliferation of human normal peripheral blood lymphocytes have been investigated. Mitogen-stimulated human lymphocytes were assayed in vitro by measuring /sup 3/H-thymidine (/sup 3/H-TdR) incorporation in the presence and in the absence of 15-30 /mu/M 3-carbethoxypsoralen (3-CPs), trimethylangelicin (TMA) and psoralen (PSR) with and without UV-A irradiation. The three furocoumarins differ in their ability to form mono- and bi-functional adducts with DNA pyrimidine furocoumarin doses and short times of UV-A irradiation used in the present study, 3-CPs did not affect /sup 3/H-TdR incorporation in PHA-stimulated human lymphocytes. TMA strongly inhibited /sup 3/H-TdR incorporation, while, unexpectedly, PSR increased /sup 3/H-TdR incorporation in the absence of irradiation, likely acting, under these experimental conditions, as a co-mitogen.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Padua (Italy)
OSTI Identifier:
5857985
Resource Type:
Journal Article
Journal Name:
Life Sci.; (United States)
Additional Journal Information:
Journal Volume: 44:26
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COUMARIN; BIOLOGICAL EFFECTS; LYMPHOCYTES; CELL PROLIFERATION; DNA ADDUCTS; IN VITRO; MAN; PSORALEN; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ULTRAVIOLET RADIATION; ADDUCTS; ANIMAL CELLS; ANIMALS; ANTICOAGULANTS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; COUMARINS; DRUGS; ELECTROMAGNETIC RADIATION; ESTERS; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LACTONES; LEUKOCYTES; MAMMALS; MATERIALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PRIMATES; PYRANS; PYRIMIDINES; RADIATIONS; RIBOSIDES; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Arslan, P, Cantini, M, Cossarizza, A, Franceschi, C, and Dall'acqua, F. Diverse effects of three furocoumarins on human lymphocyte proliferation. United States: N. p., 1989. Web. doi:10.1016/0024-3205(89)90357-3.
Arslan, P, Cantini, M, Cossarizza, A, Franceschi, C, & Dall'acqua, F. Diverse effects of three furocoumarins on human lymphocyte proliferation. United States. doi:10.1016/0024-3205(89)90357-3.
Arslan, P, Cantini, M, Cossarizza, A, Franceschi, C, and Dall'acqua, F. Sun . "Diverse effects of three furocoumarins on human lymphocyte proliferation". United States. doi:10.1016/0024-3205(89)90357-3.
@article{osti_5857985,
title = {Diverse effects of three furocoumarins on human lymphocyte proliferation},
author = {Arslan, P and Cantini, M and Cossarizza, A and Franceschi, C and Dall'acqua, F},
abstractNote = {The biological effects of three furocoumarins on the proliferation of human normal peripheral blood lymphocytes have been investigated. Mitogen-stimulated human lymphocytes were assayed in vitro by measuring /sup 3/H-thymidine (/sup 3/H-TdR) incorporation in the presence and in the absence of 15-30 /mu/M 3-carbethoxypsoralen (3-CPs), trimethylangelicin (TMA) and psoralen (PSR) with and without UV-A irradiation. The three furocoumarins differ in their ability to form mono- and bi-functional adducts with DNA pyrimidine furocoumarin doses and short times of UV-A irradiation used in the present study, 3-CPs did not affect /sup 3/H-TdR incorporation in PHA-stimulated human lymphocytes. TMA strongly inhibited /sup 3/H-TdR incorporation, while, unexpectedly, PSR increased /sup 3/H-TdR incorporation in the absence of irradiation, likely acting, under these experimental conditions, as a co-mitogen.},
doi = {10.1016/0024-3205(89)90357-3},
journal = {Life Sci.; (United States)},
number = ,
volume = 44:26,
place = {United States},
year = {1989},
month = {1}
}