L-( sup 3 H) glutamate binding to a membrane preparation from the optic lobe of the giant freshwater prawn Macrobrachium rosenbergii de Man

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90421-7· OSTI ID:5857879

Pratumtan, P; Govitrapong, P; Withyachumnarnkul, B; Poolsanguan, B ^[1]

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1. Mahidol Univ., Bangkok (Thailand) Mahidol Univ., Nakorn Pathom (Thailand)

Membrane preparation from the optic lobe of the giant freshwater prawn, Macrobrachium rosenbergii de Man, was examined for the presence of specific L-({sup 3}H) glutamate binding. The optic lobes were isolated from live animals. The tissue was homogenized and the membrane fraction isolated by differential centrifugation. The membrane suspension was incubated with 10-1,000 nM of L-({sup 3}H) glutamate at 37{degree}C for 60 min. Nonspecific binding was determined by incubating the mixture with 100 {mu}M L-glutamate. L-({sup 3}H) glutamate specifically bound to the membrane fraction with a dissociation equilibrium constant (Kd) of 205 nM and maximum number of binding sites (Bmax) of 2.04 n mol/mg protein. By using LIGAND computerized program, the saturation isotherm binding pattern indicates a single type of binding. To determine the type of glutamate receptors, competitive inhibition and IC{sub 50} of several glutamate agonists and antagonists were determined. The study reveals a metabotropic type of binding site.

• OSTI ID: 5857879
• Journal Information: Life Sciences; (USA), Vol. 48:1; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
L CODES
GLUTAMIC ACID
CELL MEMBRANES
CENTRIFUGATION
CRUSTACEANS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COMPUTER CODES
HYDROGEN COMPOUNDS
INVERTEBRATES
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques