Down-regulation of endothelin receptors in the ventrolateral medulla of spontaneously hypertensive rats

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90460-S· OSTI ID:5857669

Gulati, A; Rebello, S ^[1]

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1. Univ. of Illinois, Chicago (USA)

The binding of ({sup 125}I) sarafotoxin 6b (SRT 6b) and ({sup 125}I) endothelin-1 (ET-1) to endothelin (ET) receptors of neuronal membranes prepared from cerebral cortex and ventrolateral medulla of 8 week old spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats was determined. ({sup 125}I) SRT 6b bound to the membranes of cerebral cortex and ventrolateral medulla at a single high affinity site. The binding of ({sup 125}I) SRT 6b in the cerebral cortex was found to be similar in SHR and WKY rats. However, in the ventrolateral medulla ({sup 125}I) SRT 6b binding was found to be significantly lower in SHR as compared to WKY rats. The decreased binding was due to decrease (48%) in the B{sub max} values in SHR rats as compared to WKY rats. The K{sub d} values were similar in SHR and WKY rats. ({sup 125}I) ET-1 also bound to the membranes of cerebral cortex and ventrolateral medulla at a single high affinity site. The binding of ({sup 125}I) ET-1 in the cerebral cortex was found to be similar in SHR and WKY rats. However, in the ventrolateral medulla ({sup 125}I) ET-1 binding was found to be significantly lower in SHR as compared to WKY rats. The decreased binding was due to 36% decrease in the B{sub max} values in SHR rats as compared to WKY rats. The K{sub d} values were similar in SHR and WKY rats. It is concluded that the population of ET receptors is less in the ventrolateral medulla of SHR rats and may be contributing to the regulation of blood pressure.

• OSTI ID: 5857669
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:12; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL VARIABILITY
BLOOD PRESSURE
BODY
BRAIN
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GENETIC VARIABILITY
HYPERTENSION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VASOCONSTRICTORS
VERTEBRATES