Chromium reduction in Pseudomonas putida
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:5857429
- Univ. of Illinois, Chicago (USA)
Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a K{sub m} of 40 {mu}M CrO{sub 4}{sup 2{minus}}. Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.
- OSTI ID:
- 5857429
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:7; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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