Solubilization of glycoproteins of envelope viruses by detergents
The action of a number of known ionic and nonionic detergents, as well as the new nonionic detergent MESK, on envelope viruses was investigated. It was shown that the nonionic detergents MESK, Triton X-100, and octyl-..beta..-D-glucopyranoside selectively solubilize the outer glycoproteins of the virus particles. The nonionic detergent MESK has the mildest action. Using MESK, purified glycoproteins of influenza, parainfluenza, Venezuelan equine encephalomyelitis, vesicular stomatitis, rabies, and herpes viruses were obtained. The procedure for obtaining glycoproteins includes incubation of the virus suspension with the detergent MESK, removal of subvirus structures by centrifuging, and purification of glycoproteins from detergents by dialysis. Isolated glycoproteins retain a native structure and biological activity and possess high immunogenicity. The detergent MESK is promising for laboratory tests and with respect to the production of subunit vaccines.
- Research Organization:
- D.I. Ivanovskii Institute of Virology, Moscow, USSR
- OSTI ID:
- 5856548
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:5; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
AGGLUTININS
ANTIBODIES
BIODEGRADATION
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DECOMPOSITION
DETERGENTS
DISSOLUTION
ELECTROPHORESIS
EMULSIFIERS
GLUCOPROTEINS
HEMAGGLUTININS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MATERIALS TESTING
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOACTIVITY
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SACCHARIDES
SOLUBILITY
SURFACTANTS
TESTING
TRACER TECHNIQUES
VIRUSES
WETTING AGENTS