Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Environ. Manage.; (United States)

DOI:https://doi.org/10.1002/em.2860060615· OSTI ID:5856133

Poirier, M C

The use of antibodies to detect chemical carcinogen-induced DNA damage involves quantitative determination and morphological localization utilizing antisera specific for carcinogen-DNA adducts. In recent years a large number of polyclonal and monoclonal antisera have been produced against individual adducts, and modified DNAs with addition products varying in structure from ethyl and methyl groups to aromatic amines, polycyclic aromatic hydrocarbons, aflatoxins, and platinum-ammine complexes. This review focuses on data generated during the past 3 yr utilizing this immunotechnology as a tool to probe mechanisms of carcinogen-DNA interactions in various model systems and in the human population. Areas discussed in depth include quantitative and morphological studies involving detection of 2-acetylaminofluorene-DNA adducts in rat liver, O/sup 6/-ethyl and O/sup 6/-methyl deoxyguanosine adducts in rat brain, benzo(a)pyrene-DNA adducts in mouse skin and cis-diamminedichloroplatinum (II)-DNA adducts in peripheral nucleated blood cells of cancer patients.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 5856133
• Journal Information: Environ. Manage.; (United States), Vol. 6:6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOGENS
RADIOIMMUNOASSAY
DNA ADDUCTS
IMMUNE SERUMS
IMMUNOLOGY
IODINE ISOTOPES
BENZOPYRENE
BRAIN
CONDENSED AROMATICS
LEUKOCYTES
LIVER
MAN
NUCLEOSIDES
PLATINUM COMPOUNDS
RATS
SKIN
ADDUCTS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIOASSAY
RIBOSIDES
RODENTS
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics