A simple method for the separation and quantitation of radiolabeled thyroid hormones in thyroxine clearance studies

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Journal of Pharmacological Methods; (USA)

DOI:https://doi.org/10.1016/0160-5402(90)90028-J· OSTI ID:5855624

Grossman, S J ^[1]

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1. Merck Sharp Dohme Research Laboratories, West Point, PA (USA)

A method was developed to facilitate the separation and quantitation of radiolabeled thyroxine in plasma for thyroxine clearance studies. Following intravenous injection of radioactive thyroxine, the radiolabeled thyroid hormones were isolated from plasma protein and polar metabolites by solid phase extraction on a C18 sorbent bed. The individual thyroid hormones were then separated by ion-pair reversed phase chromatography and sequentially eluted through a UV detector and radiochromatographic detector. The radioactivity of individual radiolabeled thyroid hormones was corrected for recovery of carrier as determined from UV absorbance. The recoveries of thyroxine and 3,5,3'-triiodothyronine (T3) were 96% and 101%, respectively.

• OSTI ID: 5855624
• Journal Information: Journal of Pharmacological Methods; (USA), Journal Name: Journal of Pharmacological Methods; (USA) Vol. 24:3; ISSN 0160-5402; ISSN JPMED
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
HORMONES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
SEPARATION PROCESSES
SPECTRA
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
ULTRAVIOLET SPECTRA