Mechanism of energy conversion and transfer in bioluminescence. Final report. [Sea pansy Renilla reniformis]
Technical Report
·
OSTI ID:5852468
Bioluminescence in the sea pansy, Renilla reniformis, a marine anthozoan coelenterate, is a complex process involving the participation of three proteins specific to anthozoan coelenterate-type systems. These are: (1) the luciferin binding protein, (2) the enzyme luciferase, and (3) the green-fluorescent protein. Each of these have been purified and characterized and the structure of luciferin has been confirmed by synthesis. Luciferin binding protein (BP-LH/sub 2/) is a specific substrate binding protein which binds one molecule of coelenterate-type luciferin per molecule of protein and which then releases luciferin in the presence of Ca/sup + +/. Luciferase is the enzyme which catalyzes oxidation (by O/sub 2/) of coelenterate-type luciferin, leading to the production of CO/sub 2/ and enzyme-bound, excited-state oxyluciferin. Oxyluciferin may then emit blue light by a direct de-excitation pathway or may transfer excitation energy to the green-fluorescent protein (GFP). GFP is a non-catalytic accessory protein which accepts excitation energy from oxyluciferin, by radiationless energy transfer, and then emits green bioluminescence. The Renilla bioluminescence system is thus the first radiationless energy transfer system the individual components of which have been purified to homogeneity, characterized, and then reassembled in vitro with restoration of the energy transfer function.
- Research Organization:
- Georgia Univ., Athens (USA). Dept. of Biochemistry
- OSTI ID:
- 5852468
- Report Number(s):
- SRO-635-7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOCHEMISTRY
BIOLOGICAL MODELS
CHEMISTRY
CONVERSION
ENERGY CONVERSION
ENZYMES
LUCIFERASE
LUCIFERIN
LUMINESCENCE
ORGANIC COMPOUNDS
OXIDASES
OXIDOREDUCTASES
PLANTS
PROTEINS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOCHEMISTRY
BIOLOGICAL MODELS
CHEMISTRY
CONVERSION
ENERGY CONVERSION
ENZYMES
LUCIFERASE
LUCIFERIN
LUMINESCENCE
ORGANIC COMPOUNDS
OXIDASES
OXIDOREDUCTASES
PLANTS
PROTEINS