Effect of vitamin D/sub 3/ metabolites on membrane proteins of chick duodenal brush borders

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(76)90364-7· OSTI ID:5852372

Moriuchi, S; Deluca, H F

Chick intestinal brush border proteins were examined by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate. Following injection of 25-hydroxyvitamin D/sub 3/ and 1,25-dihydroxyvitamin D/sub 3/, a large molecular weight protein present in the vitamin D-deficient brush borders diminishes and a larger protein appears. This change occures before calcium binding protein can be detected by Chelex assay and prior to the increase in total alkaline phosphatase but correlates closely with increased intestinal calcium absorption in response to the metabolites. The two brush border proteins have been solubilized with n-butanol and partially characterized. The vitamin D-deficient protein has a molecular weight of about 200,000 and has alkaline phosphatase activity but no detectable calcium binding activity. The protein which appears in response to metabolites has a molecular weight of 230,000 binds calcium, and also has alkaline phosphatase activity.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5852372
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 174; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ALKALINE PHOSPHATASE
ANIMALS
BINDING ENERGY
BIRDS
BODY
CALCIUM
CELL CONSTITUENTS
CELL MEMBRANES
CHICKENS
CHOLECALCIFEROL
DIGESTIVE SYSTEM
ELEMENTS
ENERGY
ENZYMES
EPITHELIUM
ESTERASES
FOWL
GASTROINTESTINAL TRACT
HYDROLASES
INTESTINAL ABSORPTION
INTESTINES
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PROTEINS
STIMULATION
TISSUES
UPTAKE
VERTEBRATES
VITAMIN D
VITAMINS