Constitutive nitrate reductase expression and inhibition in winged bean

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5851646

Wu, Shenchuan; Harper, J E ^[1]

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1. Univ. of Illinois, Urbana (USA)

It was found that NO{sub 3}{sup {minus}} had no effect on winged bean nitrate reductase activity (NRA). Similar NRA was expressed in plants grown on NO{sub 3}{sup {minus}}, urea, NH{sub 4}{sup +}, and nil N. This indicated that the primary NR expressed in winged bean was constitutive, rather than substrate-inducible. Maximum NRA in winged bean was obtained in the light. KClO{sub 3} was capable of inhibiting NRA of leaves if added to the root growth medium or to the NR assay medium, indicating possible competition with NO{sub 3}{sup {minus}} at the reduction site. While it has previously been shown that either cycloheximide alone, or both cycloheximide and chloramphenicol impair the synthesis of NR protein, our data unexpectedly demonstrated that cycloheximide had little effect on NRA, whereas chloramphenicol greatly inhibited the expression of NRA in winged bean. One interpretation is that chloroplasts may influence the activity and/or synthesis of constitutive NR proteins.

• OSTI ID: 5851646
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
NITROGEN OXIDES
BIOCHEMICAL REACTION KINETICS
ENZYME ACTIVITY
OXIDOREDUCTASES
PHASEOLUS
CHALCOGENIDES
ENZYMES
KINETICS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
REACTION KINETICS
550200* - Biochemistry
540210 - Environment
Terrestrial- Basic Studies- (1990-)