Comparative measurements of potassium and chloride with ion-sensitive microelectrodes and x-ray microanalysis in cultured skeletal muscle fibers

Journal Article · Tue Jan 01 04:00:00 EST 1985 · In Vitro Cell. Develop. Biol.; (United States)

DOI:https://doi.org/10.1007/BF02620913· OSTI ID:5850267

Acker, H; Pietruschka, F; Zierold, K

Data of the intracellular electrolyte concentration of potassium and chloride in cultured muscle cells measured by x-ray analysis were compared by using the different activity coefficients with intracellular potassium and chloride activities measured with double-barrelled microelectrodes. By using an activity coefficient of 0.6, 95% of the potassium microelectrode measurements are in accordance with the x-ray analysis values, in spite of a scattering of the values. Membrane potential and intracellular potassium values are linearly related. x-ray analysis and ion-sensitive microelectrodes measured the cytoplasmic chloride in the same range. Taking into account known activity coefficients, an error of 25% must be assumed with the intracellular chloride measurements. However, x-ray analysis and ion-sensitive microelectrode investigations are reliable tools to study intracellular potassium and chloride changes, which play an important role in membrane characteristics.

• Research Organization: Max-Planck Institut fuer Systemphysiologie, Dortmund, Germany, F.R.
• OSTI ID: 5850267
• Journal Information: In Vitro Cell. Develop. Biol.; (United States), Journal Name: In Vitro Cell. Develop. Biol.; (United States) Vol. 1; ISSN ICDBE
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ANIMAL CELLS
ANIMALS
CHEMICAL ANALYSIS
CHLORIDES
CHLORINE COMPOUNDS
COHERENT SCATTERING
DIFFRACTION
ELECTRON MICROPROBE ANALYSIS
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
MAMMALS
METALS
MICROANALYSIS
MUSCLES
POTASSIUM
RATS
RODENTS
SCATTERING
SOMATIC CELLS
VERTEBRATES
X-RAY DIFFRACTION