Three classes of abscisic acid (ABA)-insensitive mutations of Arabidopsis define genes that control overlapping subsets of ABA responses

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.1172· OSTI ID:5850092

Finkelstein, R R ^[1]; Somerville, C R ^[2]

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1. Univ. of California, Santa Barbara (USA)
2. Michigan State Univ., East Lansing (USA)

Wild type and three absicisic acid (ABA)-insensitive mutants of Arabidopsis (ABI1, ABI2, and ABI3) were compared for their ability to respond to ABA for a variety of ABA-inducible responses throughout the life cycle of the plants. The responses tested included effects on seedling growth, proline accumulation in seedlings, ABA-regulated protein synthesis in plantlets, and seed storage protein and lipid synthesis and accumulation. The abi1 and abi2 mutants showed reduced sensitivity to ABA for inhibition of seedling growth, induction of proline accumulation, and alterations in protein synthesis patterns during vegetative growth, but had wild type levels of storage reserves. In contrast, the abi3 mutant had wild type sensitivity for induction of proline accumulation and was only slightly less responsive to ABA with respect to effects on seedling growth and changes in patterns of protein synthesis. The major effects of this mutation were on seed development. Seeds of the abi3 mutant had two-thirds of the wild type level of storage protein and one-third the wild type level of eicosenoic acid, the major fatty acid component of storage lipids in wild type seeds. These results show that none of the abi mutants is insensitive for all ABA-inducible responses and that the abi3 effects are not seed-specific. Comparison of the degree of ABA sensitivity of monogenic mutant lines with that of digenic mutant lines carrying pairwise combinations of the abi mutations suggests that ABA responses in mature seeds are controlled by at least two parallel pathways.

• OSTI ID: 5850092
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSCISIC ACID
AMINES
AMINO ACIDS
AUXINS
AZOLES
BIOSYNTHESIS
CARBOXYLIC ACIDS
GENES
GROWTH
HERBS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LIPIDS
METABOLISM
MONOCARBOXYLIC ACIDS
MUTANTS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYSIOLOGY
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANTS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
SEEDLINGS
SENSITIVITY
SYNTHESIS